Recombinant Human SUMF1 Protein (His Tag) (PKSH033084)
For research use only.
| Synonyms | C-Alpha-Formylglycine-Generating Enzyme 1, FGE, SUMF1, Sulfatase-Modifying Factor 1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ser34-Asp374 |
| Accession | Q8NBK3 |
| Calculated Molecular Weight | 38.4 kDa |
| Observed Molecular Weight | 38-42 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 2mM CaCl2, 10% Glycerol, pH 7.5. |
| Reconstitution | Not Applicable |
| Background | Human Sulfatase Modifying Factor 1 (SUMF1) is a 42kDa protein. SUMF1 is a Ca2+-binging member of the sulfatase-modifying factor family. SUMF1 is a soluble ER lumenal glycoprotein, it converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, SUMF1 can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF2. Three splice isoforms are known. |
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