Recombinant Human SULT1B1 Protein (His Tag) (PKSH031074)
For research use only.
| Synonyms | ST1B1, ST1B2, SULT1B1, SULT1B2, Sulfotransferase 1B1, Sulfotransferase 1B2, Sulfotransferase Family Cytosolic 1B Member 1, Thyroid Hormone Sulfotransferase |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Leu 2-Ile 296 |
| Accession | NP_055280.2 |
| Calculated Molecular Weight | 35.7 kDa |
| Observed Molecular Weight | 34 kDa |
| Tag | N-His |
| Bio-activity | Measured by its ability to transfer sulfate from PAPS to 1-Napthol. The specific activity is > 40 pmoles/min/μg. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile 20mM Tris, 0.1 M NaCl, 10% glycerol, 1mM DTT, pH 8.0 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Sulfotransferase family cytosolic 1B member 1; also known as Sulfotransferase 1B1; Sulfotransferase 1B2; Thyroid hormone sulfotransferase; SULT1B1 and ST1B2; is a cytoplasm protein which belongs to thesulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones; neurotransmitters; drugs; and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT1B1 is highly expressed in the liver; peripheral blood leukocytes; colon (mucosal lining); small intestine (jejunum) and spleen. A lesser expression of SULT1B1 was observed in the lung; placenta and thymus. SULT1B1 catalyzes the sulfate conjugation of many hormones; neurotransmitters; drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds; and therefore their renal excretion; but it can also result in bioactivation to form active metabolites. SULT1B1 sulfates dopamine; small phenols such as 1-naphthol and p-nitrophenol and thyroid hormones; including 3;3'-diiodothyronine; triidothyronine; reverse triiodothyronine and thyroxine. |
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