Recombinant Human ST8SIA1 Protein (His Tag) (PKSH033251)
For research use only.
| Synonyms | 8-Sialyltransferase, 8-Sialyltransferase 8A, Alpha-2, Alpha-N-Acetylneuraminide Alpha-2, Ganglioside GD3 Synthase, Ganglioside GT3 Synthase, SIAT8, SIAT8-A, SIAT8A, ST8SIA1, ST8SiaI, Sialyltransferase 8A, Sialytransferase St8Sia I |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Tyr49-Ser356 |
| Accession | Q92185 |
| Calculated Molecular Weight | 36.2 kDa |
| Observed Molecular Weight | 48 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
| Reconstitution | Not Applicable |
| Background | α-N-Acetylneuraminide α-2,8-Sialyltransferase (ST8SIA1) belongs to the glycosyltransferase 29 family. ST8SIA1 is a sialytransferase that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce GD3 and GT3. ST8SIA1 is highly expressed in melanoma cell lines, adult and fetal brain, low expressed in adult and fetal lung. ST8SIA1 may act as a type II transmembrane protein with a short N-termianl cytoplasmic domain and a single-pass transmembrane domain folowed by an enzymatic domain in the lument of the Golgi apparatus. |
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