Recombinant Human Serum Albumin/HSA/ALB Monoclonal Antibody (AN300577P)
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For research use only.
| Verified Samples |
Verified Samples in WB:?HepG2 Verified Samples in IF: HepG2 Verified Samples in FCM: HepG2 Verified Samples in IP: HepG2 |
| Dilution | WB 1:500-1:2000, ICC/IF 1:20-1:100, FCM 1:25-1:100, IP 0.5-2 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, FCM, ICC/IF, IP |
| Clonality | Monoclonal |
| Immunogen | Human Serum Albumin/HSA |
| Abbre | ALB |
| Synonyms | UNQ, PRO, ALB, HSA, PRO0883, PRO0903, PRO1341, albumin, Alb1, Alb-1, HAS, mAlb, Serum albumin, UMA, GIG20, GIG42, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696, ALBU, Albumin (32 AA), Albumin (AA 34), cell growth inhibiting protein 42, DKFZp779N1935, growth-inhibiting protein 20, OTTHUMP00000220436, OTTHUMP00000220438, OTTHUMP00000220439, UNQ696/PRO1341 |
| Swissprot | |
| Calculated MW | 68 kDa |
| Observed MW |
68 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Plasma. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Stem Cells, Developmental Biology, Kits, Lysates, Other, Metabolism |
| Clone No. | 11A4 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. |
Other Clones
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Unconjugated
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