Recombinant Human SEPHS1 Protein (His Tag) (PKSH033016)
For research use only.
| Synonyms | SELD, SEPHS1, SPS, SPS1, Selenide, Selenium donor protein 1, Selenophosphate synthase 1, water dikinase 1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Ser392 |
| Accession | P49903 |
| Calculated Molecular Weight | 43.9 kDa |
| Observed Molecular Weight | 42 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 100mM glycine, 10% Glycerol, pH 7.3. |
| Reconstitution | Not Applicable |
| Background | Selenophosphate synthetase 1 (SEPHS1) belongs to the selenophosphate synthase 1 family, Class II subfamily. It has four different isoforms by alternative splicing. Isoform 1 and isoform 2 are gradually expressed during the cell cycle until G2/M phase and then decreased, which Isoform 3 is gradually expressed during the cell cycle until S phase and then decreased. SEPHS1 can be activated by phosphate ions and by potassium ions. It can synthesize synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. |
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