Recombinant Human PSMA Protein (His Tag) (PKSH033667)
For research use only.
| Synonyms | Cell growth-inhibiting gene 27 protein, FGCP, FOLH, FOLH1, Folate hydrolase 1, GCP2, GCPII, Glutamate carboxypeptidase 2, Mgcp, NAALAD1, NAALADase I, NAALAdase, PSM, PSMA, mGCP |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Lys44-Ala750 |
| Accession | Q04609 |
| Calculated Molecular Weight | 80.6 kDa |
| Observed Molecular Weight | 90-120 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 5% Trehalose, pH 5.5. |
| Reconstitution | Not Applicable |
| Background | Glutamate carboxypeptidase 2, also known as FOLH1, PSMA, belongs to the M28B subfamily and the peptidase M28 family. It is highly expressed in prostate epithelium and can be detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). PSMA is used as a diagnostic and prognostic indicator of prostate cancer, and as a possible marker for various neurological disorders such as schizophrenia, Alzheimer disease and Huntington disease. It has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity and has a preference for tri-alpha-glutamate peptides. PSMA involves in prostate tumor progression and also exhibits a dipeptidyl-peptidase IV type activity. In vitro, PSMA cleaves Gly-Pro-AMC. PSMA is stable at pH greater than 6.5. |
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