Recombinant Human POSTN Protein (His Tag) (PDEH100684)
For research use only.
| Synonyms | Fasciclin-I like, MGC119510, MGC119511, OSF 2, OSF-2, OSF2, Osteoblast specific factor, Osteoblast specific factor 2, Osteoblast specific factor 2 (fasciclin I like), Osteoblast-specific factor 2, PDLPOSTN, Periodontal ligament specific periostin, Periostin |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala21-Leu365 |
| Accession | Q15063-1 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 40.3 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Purity | > 85% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | Periostin (POSTN,PN),originally named as osteoblast-specific factor 2 (OSF-2),is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues,and has a role in bone,tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types,includidng lung,ovary,breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour,and it is required to allow cancer stem cell maintenance. |
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