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Recombinant Human PFK1/PFKM Protein (His & GST Tag) (PKSH030320)

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50μg $ 680.00
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For research use only.

Synonyms 6-phosphofructokinase, ATP-PFK, GSD7, PFK-1, PFK1, PFKA, PFKM, PFKX, PPP1R122, Phosphofructo-1-kinase isozyme A, Phosphofructokinase 1, Phosphohexokinase, muscle type
Species Human
Expression Host Baculovirus-Insect Cells
Sequence Thr 2-Val 780
Accession P08237-1
Calculated Molecular Weight 112.9 kDa
Observed Molecular Weight 113 kDa
Tag N-His-GST
Bio-activity Not validated for activity
Purity > 90 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol
Reconstitution Not Applicable
Background PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related with glycogen storage disease type VII, also identified as Tarui disease.
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