Recombinant Human Neurocalcin-δ/NCALD Protein (His Tag) (PKSH032796)
For research use only.
| Synonyms | NCALD, Neurocalcin-Delta |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Phe193 |
| Accession | P61601 |
| Calculated Molecular Weight | 24.4 kDa |
| Observed Molecular Weight | 20 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 40% Glycerol, pH 8.0. |
| Reconstitution | Not Applicable |
| Background | Neurocalcin-delta (NCALD) is a neuronal calcium-binding protein that belongs to the neuronal calcium sensor (NCS) family. It expressed in mammalian brains. NCALD contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein possesses a Ca2+ /myristoyl switch. It is cytosolic at resting calcium levels. However, elevated intracellular calcium levels induce a conformational change which exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. NCALD protein is thought to be a regulator of G protein-coupled receptor signal transduction. |
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