Recombinant Human NECTIN4 Protein(Sumo Tag) (PDEH100662)
For research use only.
| Synonyms | PRR, DKFZp686K, poliovirus receptor-related, Poliovirus receptor-related protein, Processed poliovirus receptor-related protein, PVRL, EDSS, DKFZp686K05193, EDSS1, Ig superfamily receptor LNIR, Nectin 4, Nectin-4, poliovirus receptor-related 4, Poliovirus receptor-related protein 4, Processed poliovirus receptor-related protein 4, LNIR, PRR4, PVRL4, NECTIN4 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Gly32-Ser349 |
| Accession | Q96NY8 |
| Calculated Molecular Weight | 51.0 kDa |
| Observed Molecular Weight | 56 kDa |
| Tag | N-Sumo |
| Bio-activity | Not validated for activity |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
| Background | Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. The Latin word necto means “to connect”, indicating the role of nectins in Ca2+‑independent cell-cell adhesion. Nectin-4 forms homodimers in cis, followed by interactions in trans with Nectin-1 or -4. Human Nectin-4 mRNA is normally expressed in the placenta, especially in endothelial cells, while in the mouse it is found in the embryo, lung, testis and brain. Human Nectin-4 cDNA encodes 510 amino acids (aa), including a 31 aa signal sequence, a 318 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 140 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N‑terminal V-type that mediates ligand binding, and two C2-type. One Nectin‑4 isoform lacks aa 412-436 in the cytoplasmic domain (1). In many human ductal breast or non-small cell lung carcinomas, Nectin‑4 is upregulated and a soluble 43 kDa form is found in the plasma. This form is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 91%, 92%, 93%, 91% and 90% amino acid sequence homology with the corresponding regions of mouse, rat, canine, porcine and bovine Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins. These interactions organize the actin cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. In humans, mutation of Nectin-4 has been correlated with ectodermal dysplasia-syndactyly syndrome, indicating a role for Nectin-4 in human development. |
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