Recombinant Human MCEE Protein (His Tag) (PDMH100013)

For research use only.
Synonyms | DL-methylmalonyl-CoA racemase, Methylmalonyl-CoA epimerase, mitochondrial |
Species | Human |
Expression Host | HEK293 Cells |
Sequence | Gln37-Ala176 |
Accession | Q96PE7 |
Calculated Molecular Weight | 16.0 kDa |
Observed Molecular Weight | 18-20 kDa |
Tag | C-His |
Bio-activity | Not validated for activity |
Form | Lyophilized powder |
Purity | > 95% as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU/mg of the protein as determined by the LAL method |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
Background | Methylmalonyl-CoA epimerase, mitochondrial (MCEE) is an enzyme which belongs to the glyoxalase I family. It converts (S)-methylmalonyl-CoA to the (R) form, catalyses the following chemical reaction: (R)-methylmalonyl-CoA (S)-methylmalonyl-CoA. It plays an important role in the catabolism of fatty acids with odd-length carbon chains. This protein deficiency is an autosomal recessive inborn error of AA metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria can appear in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma. |
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