Recombinant Human LC3A/B Protein(Trx Tag) (PDEH100633)
For research use only.
| Synonyms | MAP1A/1BLC, MAP1 light chain 3-like protein, MAP1ALC, MAP1LC3B, ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3A/LC3B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1ALC3, Autophagy related protein LC3 A, Autophagy related ubiquitin like modifier LC3 A, Autophagy related ubiquitin like modifier LC3 B, LC3, LC3II, MAP, MAP1 light chain 3 like protein 1, MAP1 light chain 3 like protein 2, MAP1A/1B light chain 3 A, MAP1LC3B a, Microtubule-associated proteins 1A/1B light chain 3B, MLP3B |
| Species | Human |
| Expression Host | E.coli |
| Sequence | His27-Val125 |
| Accession | Q9GZQ8 |
| Calculated Molecular Weight | 30.7 kDa |
| Observed Molecular Weight | 31 kDa |
| Tag | N-Trx |
| Bio-activity | Not validated for activity |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | LC3A, also known as MAP1LC3A, is one of the light chain subunits that function together with both MAP1A and/or MAP1B. MAP1A and MAP1B are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. As a light chain subunit, MAP1LC3A has an important part in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP1LC3A is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver, and skeletal muscle but are absent in thymus and peripheral blood leukocytes. |
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