Recombinant Human KEAP1/INRF2 Protein (PKSH030943)
For research use only.
| Synonyms | INRF2, KEAP-1, KLHL19 |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Gln2-Cys624 |
| Accession | Q14145 |
| Calculated Molecular Weight | 69.7 kDa |
| Observed Molecular Weight | 64 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Kelch-like ECH-associated protein 1; also known as cytosolic inhibitor of Nrf2; Kelch-like protein 19; KEAP1 and INRF2; is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain; one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed; with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor; which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions; KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome; thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome. |
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