Recombinant Human IL18 protein (Avi,His Tag) (PDEH100869)
For research use only.
| Synonyms | MGC, IL1F, IL-1F, IL18, IGIF, IL-18, IL-1g, IL1F4, GIF, Iboctadekin, IFN-gamma-inducing factor, IL-1 gamma, IL-1F4, Interferon gamma-inducing factor, Interferon-gamma-inducing factor, Interleukin 18, Interleukin-1 gamma, Interleukin-18, MGC12320, IFN gamma inducing factor, IL 1 gamma, IL 18, IL 1g, IL1 gamma, IL18 protein, IL1g, IL1gamma, ILIF4, Interferon gamma inducing factor, Interleukin 1 gamma, Interleukin 18 (interferon-gamma-inducing factor), Interleukin18 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Tyr37-Lys115 |
| Accession | Q14116 |
| Calculated Molecular Weight | 8.6 kDa |
| Observed Molecular Weight | 12 kDa |
| Tag | C-Avi-His |
| Bio-activity | Not validated for activity |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1&minus,like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4&minus, and STAT6&minus,dependent fashion. IL-18 and T cell receptor&minus,mediated stimulation could induce naï,ve CD4+ T cells to develop into IL-4&minus,producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-&alpha, and IL-1&beta,, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA. |
| Modification | Biotinylated |
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Biotinylated
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