Recombinant Human IL-1α Protein (PDMH100025)
For research use only.
| Synonyms | IL1F, IL1A, IL-1A, IL1, IL1-ALPHA, IL1F1, BAF, FAF, Hematopoietin 1, Hematopoietin-1, IL 1 alpha, IL 1A, IL1 ALPHA, IL-1 alpha, IL1-A, Interleukin 1 alpha, Interleukin1 alpha, Interleukin-1 alpha, LAF, LEM, Preinterleukin 1 alpha, Pro interleukin 1 alpha, Pro-Interleukin-1-Alpha |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met1-Ala271 |
| Accession | P01583 |
| Calculated Molecular Weight | 30.6 kDa |
| Observed Molecular Weight | 35 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
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