Recombinant Human Grancalcin/GCA Protein (PKSH032506)
For research use only.
| Synonyms | GCA, GCL, Grancalcin |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ile217 |
| Accession | P28676 |
| Calculated Molecular Weight | 24.0 kDa |
| Observed Molecular Weight | 26 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH 8.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Grancalcin (GCA) is a cytoplasmic granule membrane protein that contains 4 EF-hand domains. GCA is calcium-binding protein and particularly abundant in human neutrophils. GCA is highly expressed in bone marrow; and it can be detected in neutrophils and macrophages. Calcium-binding protein GCA cooperates with SRI and LCP1; so it may play a role in the adhesion of neutrophils to fibronectin. GCA also may play a role in the formation of focal adhesions. |
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