Recombinant Human DDR2 Kinase/CD167b Protein (Fc Tag) (PKSH031758)
For research use only.
| Synonyms | CD167, MIG20a, NTRKR3, TKT, TYRO10 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Arg 399 |
| Accession | NP_001014796.1 |
| Calculated Molecular Weight | 69.4 kDa |
| Observed Molecular Weight | 87 kDa |
| Tag | C-hFc |
| Bio-activity | Immobilized Rat tail Collagen I at 10 μg/ml can bind recombinant human DDR2-Fc Chimera with a linear range of 2. 5-80 ng/ml. Scatchard analysis showed the affinity constant (Kd) of recombinant human DDR2-Fc Chimera bound to rat tail collagen I was 6. 8 nM. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity. |
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