Recombinant Human CXCL12 (24-88) protein(His Tag) (PKSH034169)
For research use only.
| Synonyms | IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Val 24-Leu 195 |
| Accession | P48061 |
| Calculated Molecular Weight | 8.6 kDa |
| Observed Molecular Weight | 10 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to chemoattract BaF3 cells transfected with human CXCR4.The ED50 for this effect is <0.5 ng/mL. |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile 20 mM sodium citrate, 0.1 M NaCl, pH 4.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Stromal Cell-Derived Factor-1 (SDF-1) is a chemokine member of the intercrine family. SDF1 is expressed as five isoforms that differ only in the C terminal tail. SDF1α and SDF1β are identical except for the four residues present in the C-terminus of SDF1β but absent from SDF1α. SDF1 isoforms interact with CXCR4 and CXCR7 receptors on the cell surface; and can also bind syndecan4. SDF1 is known to influence lymphopoiesis; regulate patterning and cell number of neural progenitors; and promote angiogenesis. It also enhances the survival of myeloid progenitor cells. |
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