Recombinant Human CTNNB1 Protein (PDEH100744)
For research use only.
| Synonyms | Beta-catenin, CTNNB, Catenin beta-1, OK, PRO2286, SW-cl.35 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met131-Tyr670 |
| Accession | P35222 |
| Calculated Molecular Weight | 59.0 kDa |
| Observed Molecular Weight | 59 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Purity | > 85% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. |
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