Recombinant Human COL9A1 Protein (His Tag) (PKSH033337)
For research use only.
| Synonyms | Collagen alpha-1(IX) chain, DJ149L1.1.2, DJ149L1.1.2 , EDM6, MED, STL4 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ala24-Pro328 |
| Accession | P20849-3 |
| Calculated Molecular Weight | 33.8 kDa |
| Observed Molecular Weight | 38-50 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | COL9A1; which is short for Collagen alpha-1(IX) chain; is a 921 aa. protein. It is a secreted protein; and exists in extracellular space and extracellular matrix. This protein is a heterotrimer of an alpha 1(IX); an alpha 2(IX) and an alpha 3(IX) chain.Each subunit is composed of three triple-helical domains interspersed with non-collagenous domains. The globular domain at the N-terminus of type IX collagen molecules represents the NC4 domain which may participate in electrostatic interactions with polyanionic glycosaminoglycans in cartilage. It ia a structural component of hyaline cartilage and vitreous of the eye. |
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