Recombinant Human CD3E & CD3G Heterodimer Protein (PKSH030476)
For research use only.
| Synonyms | CD3E, CD3G Heterodimer, IMD18, T3E, TCRE |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Asp126&Met1-Ser116 |
| Accession | NP_000724.1&NP_000064.1 |
| Calculated Molecular Weight | 40.1&37.1 kDa |
| Observed Molecular Weight | 42-47 kDa |
| Tag | C-hFc-Flag & mFc Tag |
| Bio-activity | Measured by its ability to bind OKT3-mIgG2a in a functional ELISA. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. |
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