Recombinant Human CA199(FUT3) Protein (His Tag) (PDEH100706)
For research use only.
| Synonyms | Lewis FT, Blood group Lewis alpha-4-fucosyltransferase, FT3B, FUT3, FucT-III, Fucosyltransferase 3, Fucosyltransferase III, Galactoside 3(4)-L-fucosyltransferase, LE |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Val35-Thr361 |
| Accession | P21217-1 |
| Calculated Molecular Weight | 38.1 kDa |
| Observed Molecular Weight | 40.9 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Purity | > 80% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination, Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. |
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