Recombinant Human BMP-2 Protein(Trx Tag) (PDEH100510)

For research use only.
Synonyms | BDA2, BMP-2, BMP-2A, Bmp2, BMP2, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A |
Species | Human |
Expression Host | E.coli |
Sequence | Gln283-Arg396 |
Accession | P12643 |
Calculated Molecular Weight | 32.4 kDa |
Observed Molecular Weight | 32 kDa |
Tag | N-Trx |
Bio-activity | Not validated for activity |
Purity | > 90% as determined by reducing SDS-PAGE. |
Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
Background | Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer, 2 monomers then associate to form the active 30 kDa homodimer,which binds to its receptor. There is also a 40-45 kDa form of BMP2,as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway,TGF beta signaling pathway,and cytokine-cytokine receptor interaction. |
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