Recombinant Human BCAS2/DAM1 Protein (His & T7 Tag) (PKSH032111)
For research use only.
| Synonyms | BCAS2, Breast Carcinoma-Amplified Sequence 2, DAM1, DNA Amplified in Mammary Carcinoma 1 Protein, Pre-mRNA-Splicing Factor SPF27, Spliceosome-Associated Protein SPF 27 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala2-Phe225 |
| Accession | O75934 |
| Calculated Molecular Weight | 28.6 kDa |
| Observed Molecular Weight | 60 kDa |
| Tag | N-T7 & C-His |
| Bio-activity | Not validated for activity |
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, 2mM DTT, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Breast Carcinoma-Amplified Sequence 2 (BCAS2) is a member of the SPF27 family. BCAS2 is a nuclear protein and widely expressed in many rtissues. BCAS2 is identified as being overexpressed in various breast cancer cell lines. BCAS2 is a component of the spliceosome, taking part in the removal of introns from mRNA precursors. BCAS2 interacts with estrogen receptor alpha and beta, thyroid hormone receptor beta, peroxisome proliferator-activated receptor gamma. BCAS2 functions as an ER co-activator and is capable of enhancing ER-mediated transcription. |
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