Recombinant HSP70/HSPA1A Monoclonal Antibody (AN300407P)
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For research use only.
| Verified Samples |
Verified Samples in WB:?A549, HCT116, Hela, HepG2, HL60, A431, MCF7, K562, C6, 293T, MOLT4, Jurkat Verified Samples in IHC: Human hepatoma, cynomolgus macaque testis Verified Samples in IF: Hela Verified Samples in FCM: HeLa Verified Samples in IP: Hela |
| Dilution | WB 1:500-1:2000, IHC-P 1:500-1:2000, ICC/IF 1:20-1:100, FCM 1:25-1:100, IP 1-4 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, FCM, ICC/IF, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human HSP70/HSPA1A protein |
| Abbre | HSPA1A |
| Synonyms | HSX, HSPA, HEL-S, HSP, HEL-S-103, HSP70-1, HSP70-1A, HSP70.1, HSP70I, HSP72, HSPA1, heat shock protein family A (Hsp70) member 1A, heat shock protein family A (Hsp70) member 1B, HSP70.2, HSP70-1B, HSP70-2, HSPA1A, Hsp70, Heat shock 70 kDa protein 1A, HSX70, heat shock protein family A (Hsp70) member 1B, HSP70.1, HSP70.2, HSP70-1, HSP70-1A, HSP70-1B, HSP70-2, HSP70I, HSP72, HSPA1 |
| Swissprot | |
| Calculated MW | 70 kDa |
| Observed MW |
70 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Secreted |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Cancer |
| Clone No. | 9D15 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity． |
Other Clones
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Unconjugated
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