Recombinant HIF-1 alpha/HIF1A Monoclonal Antibody (AN300409P)
For research use only.
| Verified Samples | Verified Samples in IF: Hela |
| Dilution | ICC/IF 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | ICC/IF |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human HIF-1 alpha/HIF1A Protein |
| Abbre | HIF1A |
| Synonyms | HIF, MOP, PASD, member of PAS protein, hypoxia inducible factor, bHLHe, HIF-1A, HIF1-ALPHA, HIF-1alpha, HIF1A, bHLHe78, HIF1, MOP1, PASD8, HIF-1-alpha, hypoxia inducible factor 1, alpha subunit, basic helix-loop-helix transcription factor, hypoxia-inducible factor 1-alpha, HIF 1alpha, member of PAS protein 1, ARNT interacting protein, ARNT-interacting prot, alpha subunit (basic helix loop helix transcription factor), ARNT-interacting protein, Basic helix loop helix PAS protein MOP1, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, HIF 1A, HIF1 A, HIF1 Alpha, Hypoxia inducible factor 1 alpha, Hypoxia inducible factor 1 alpha isoform I.3, Hypoxia inducible factor 1 alpha subunit, Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor, Hypoxia inducible factor1alpha, Member of PAS superfamily 1, Member of the PAS Superfamily 1, MOP 1, PAS domain-containing protein 8, PASD 8 |
| Swissprot | |
| Calculated MW | 111 kDa |
| Observed MW |
111 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus, Cytoplasmic in normoxia, nuclear translocation in response to hypoxia |
| Tissue Specificity | Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Epigenetics and Nuclear Signaling, Cancer, Metabolism |
| Clone No. | 5D7 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha. |
Other Clones
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