Recombinant GSTM2 Monoclonal Antibody (AN300247P)
For research use only.
| Verified Samples |
Verified Samples in WB: 293T Verified Samples in IF: Hela Verified Samples in IP: Hela, Jurkat |
| Dilution | WB 1:500-1:2000, ICC/IF 1:20-1:100, IP 1-4 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, ICC/IF, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human GSTM2 protein |
| Abbre | GSTM2 |
| Synonyms | GST, GSTM2, GST4, GSTM, GSTM2-2, GTHMUS, Glutathione S alkyltransferase M2, Glutathione S aralkyltransferase M2, Glutathione S aryltransferase M2, Glutathione S transferase 4, Glutathione S transferase M1, Glutathione S transferase M2 (muscle), Glutathione S transferase Mu 2, Glutathione S transferase Mu 2 (muscle), glutathione S-transferase mu 2, glutathione S-transferase mu 2 (muscle), GST class mu 2, GST class-mu 2, GST muscle, MGC117303, S (hydroxyalkyl)glutathione lyase M2 |
| Swissprot | |
| Calculated MW | 26 kDa |
| Observed MW |
26 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Metabolism, Tags & Cell Markers |
| Clone No. | 6F6 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. |
Other Clones
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Unconjugated
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