Recombinant GM130 Monoclonal Antibody (AN300891L)
For research use only.
| Verified Samples | Verified Samples in WB: Hela |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human GM130 protein |
| Abbre | GM130 |
| Synonyms | GOLGA, golgin A, GOLGA2, GM130, golgin A2, 130 kDa cis Golgi matrix protein, 130 kDa cis-Golgi matrix protein, Cis golgi matrix protein GM130, Gm130 autoantigen, GOGA2, GOLGA 2, Golgi autoantigen, Golgi autoantigen golgin subfamily a 2, Golgi matrix protein GM130, Golgin 95, Golgin subfamily a 2, Golgin subfamily A member 2, Golgin-95, MGC20672, SY11 protein |
| Swissprot | |
| Calculated MW | 113 kDa |
| Observed MW |
130 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Tags & Cell Markers, Signal Transduction |
| Clone No. | 7C3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids inThe secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions betweenThe Golgi and microtubules are thought to be important forThe reorganization ofThe Golgi after it fragments during mitosis. This gene encodes one ofThe golgins, a family of proteins localized toThe Golgi. This encoded protein has been postulated to play roles inThe stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, butThe full-length nature of these variants has not been determined. |
Other Clones
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Other Formats
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Unconjugated
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