Recombinant GFAP Monoclonal Antibody (AN300975L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Mouse brain Verified Samples in IHC: Human brain tissue, Mouse brain tissue, Mouse brain |
| Dilution | IHC 1:200-1:1000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human GFAP protein |
| Abbre | GFAP |
| Synonyms | ZGC, etID36982, wu:fb34h, wu:fk42c, ALXDRD, GFAP, cb345, etID36982.3, FLJ42474, FLJ45472, gfapl, Glial Fibrillary Acidic Protein, Intermediate Filament Protein, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485, wu:fb34h11, ALXDRD, cb345, etID36982.3, FLJ42474, FLJ45472, GFAP, GFAP, gfapl, Glial fibrillary acidic protein, Intermediate filament protein, wu:fk42c12, xx:af506734, zgc:110485 |
| Swissprot | |
| Calculated MW | 50 kDa |
| Observed MW |
50 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Signal Transduction, Tags & Cell Markers, Stem Cells |
| Clone No. | 10A10 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
Other Clones
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Other Formats
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Unconjugated
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