Recombinant GFAP Monoclonal Antibody (AN300140P)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse brain |
| Dilution | WB 1:500-1:1000, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide corresponding to the center region of the human GFAP |
| Abbre | GFAP |
| Synonyms | ZGC, etID36982, wu:fb34h, wu:fk42c, ALXDRD, GFAP, cb345, etID36982.3, FLJ42474, FLJ45472, gfapl, Glial Fibrillary Acidic Protein, Intermediate Filament Protein, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485, wu:fb34h11, ALXDRD, cb345, etID36982.3, FLJ42474, FLJ45472, GFAP, GFAP, gfapl, Glial fibrillary acidic protein, Intermediate filament protein, wu:fk42c12, xx:af506734, zgc:110485 |
| Swissprot | |
| Calculated MW | 50 kDa |
| Observed MW |
45 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Signal Transduction, Tags & Cell Markers, Stem Cells |
| Clone No. | 4B2 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | GFAP (Glial fibrillary acidic protein) is a type III intermediate filament protein. It is the major component of astrocyte intermediate filament. Defects in GFAP are a cause of Alexander disease. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. At the amino acid sequence level, human GFAP shares 91% and 90% identity with rat and mouse GFAP, respectively. |
Other Clones
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Unconjugated
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