Recombinant Galectin-1/LGALS1 Monoclonal Antibody (AN300313P)
For research use only.
| Verified Samples |
Verified Samples in WB:?Hela, K562, A431 Verified Samples in IP: Hela |
| Dilution | WB 1:500-1:2000, IP 1-4 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human Galectin-1/LGALS1 protein |
| Abbre | LGALS1 |
| Synonyms | GAL, Galactoside-Binding Soluble, LGALS, LGALS1, GAL1, GBP, Galactoside-Binding Soluble 1, Lectin, 14 kDa laminin-binding protein, 14 kDa lectin, Beta galactoside binding lectin, Beta galactoside binding lectin L 14 I, beta galactoside binding protein, Beta-galactoside-binding lectin L-14-I, DKFZp686E23103, Gal 1, Gal-1, Galaptin, Galbp, Galectin, Galectin1, Galectin-1, HBL, HLBP14, HPL, L 14.5, L14, L-14.5, Lactose binding lectin 1, Lactose-binding lectin 1, Lect14, Lectin galactoside binding soluble 1, Lectin galactoside-binding soluble 1, LEG1, LGALS 1, Lgals1 lectin galactose binding soluble 1, MAPK activating protein MP12, Putative MAPK activating protein MP12, Putative MAPK-activating protein PM12, S Lac lectin 1, S-Lac lectin 1 |
| Swissprot | |
| Calculated MW | 14 kDa |
| Observed MW |
14 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Cancer |
| Clone No. | 7F15 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. |
Other Clones
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Unconjugated
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