Recombinant GAD-65/67 Monoclonal Antibody (AN301129L)
For research use only.
| Verified Samples |
Verified Samples in WB: Rat brain Verified Samples in IHC: Human brain |
| Dilution | IHC 1:200-1:1000, WB 1:10000-1:50000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human GAD-65/67 protein |
| Abbre | GAD-65/67 |
| Synonyms | GAD, GAD2, GAD65, 65 kDa glutamic acid decarboxylase, DCE 2, DCE2, GAD 2, GAD 65, GAD-2, GAD-65, Glutamate decarboxylase 2, Glutamate decarboxylase 2 (pancreas), Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa), Glutamate Decarboxylase 65, Glutamate decarboxylase 65 kDa isoform, Glutamic Acid Decarboxylase 2, Glutamic Acid Decarboxylase 65, MGC161605, MGC161607, GAD-65/67 |
| Swissprot | |
| Calculated MW | 65 kDa,67 kDa |
| Observed MW |
65 kDa,67 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Intracellular, plasma membrane, vesicle membrane, presynaptic active zone, clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Metabolism |
| Clone No. | 12G12 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | GAD1(glutamate decarboxylase 1) Homo sapiens This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. |
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Unconjugated
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