Recombinant Fibronectin Fragment 2 Monoclonal Antibody (AN300318P)
For research use only.
| Verified Samples |
Verified Samples in WB:?HepG2 Verified Samples in IP: HepG2 |
| Dilution | WB 1:500-1:2000, IP 1-4 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human Fibronectin Fragment 2 protein |
| Abbre | FN1 |
| Synonyms | DKFZp686F, DKFZp686H, DKFZp686I, DKFZp686O, FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, fibronectin, Cold insoluble globulin, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED B, Fibronectin 1, Migration stimulating factor, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3, Cold-insoluble globulin, fFN |
| Swissprot | |
| Calculated MW | 233 kDa |
| Observed MW |
250 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Tissue Specificity | Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level) |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Stem Cells, Cancer, Developmental Biology |
| Clone No. | 8D5 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. |
Other Clones
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Unconjugated
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