Recombinant Estrogen Receptor alpha Monoclonal Antibody (E-AB-81467)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, Hela Verified Samples in IF: Human breast cancer, MCF-7 |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, IF |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human Estrogen Receptor alpha |
| Abbre | Estrogen Receptor alpha |
| Synonyms | 4, 5, 6, 7*, 7*/822 isoform, 8*/941 isof, DKFZp686N23123, ER, ER alpha, ER-alpha, ESR, ESR1, ESRA, Era, Estradiol receptor, Estrogen nuclear receptor alpha, Estrogen receptor 1, Estrogen receptor alpha, Estrogen receptor alpha 3*, Estrogen receptor alpha delta 3* |
| Swissprot | |
| Calculated MW | 66 kDa |
| Observed MW |
66 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Neuroscience, Signal Transduction |
| Clone No. | R05-1E4 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. |
Other Clones
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Other Formats
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Unconjugated
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