Recombinant ERP72/PDIA4 Monoclonal Antibody (AN300439P)
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For research use only.
| Verified Samples |
Verified Samples in WB:?A431, NIH-3T3, 293T Verified Samples in IF: Hela Verified Samples in FCM: HepG2 Verified Samples in IP: Hela |
| Dilution | WB 1:500-1:2000, ICC/IF 1:20-1:100, FCM 1:25-1:100, IP 1-4 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, FCM, ICC/IF, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human ERP72/PDIA4 Protein |
| Abbre | PDIA4 |
| Synonyms | PDIA, Protein Disulfide-Isomerase A, ERP, PDIA4, ERP70, ERP72, ERp-72, Endoplasmic Reticulum Resident Protein 70, Endoplasmic Reticulum Resident Protein 72, ER Protein 70, ER Protein 72, Protein Disulfide-Isomerase A4, Calcium binding protein intestinal related, EPR70, EPR72, ERP 70, ERp 72, intestinal-related), PDIA 4, PDIR, Protein disulfide isomerase A4, Protein disulfide isomerase associated 4, Protein disulfide isomerase family A member 4, Protein disulfide isomerase related protein, protein disulfide isomerase related protein (calcium-binding protein, Protein ERp 72, Protein ERp72 |
| Swissprot | |
| Calculated MW | 73 kDa |
| Observed MW |
73 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction |
| Clone No. | 5B7 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly. |
Other Clones
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Unconjugated
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