Recombinant ERK2 Monoclonal Antibody (E-AB-81446)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, Rat Rat Brain, C6, 3T3, Hela Verified Samples in IHC: Human Cholangiocarcinoma |
| Dilution | WB 1:500-1:1000, IHC 1:20-1:100, IF 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human ERK2 |
| Abbre | ERK2 |
| Synonyms | ERK, ERK 2, ERK-2, ERT1, Extracellular Signal Regulated Kinase 2, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK2, MK01, Mapk1, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, P38 |
| Swissprot | |
| Calculated MW | 41 kDa |
| Observed MW |
41 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Neuroscience, Signal Transduction, Stem Cells |
| Clone No. | R07-1A9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a member of the MAP kinase family.MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development.The activation of this kinase requires its phosphorylation by upstream kinases.Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity.The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions.Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. |
Other Clones
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Unconjugated
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