Recombinant ER alpha/ESR1 Monoclonal Antibody (AN300296P)
For research use only.
| Verified Samples | Verified Samples in WB:?MCF7, HeLa, Molt4, Raji |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | A synthetic peptide corresponding to the center region of the Human ER alpha/ESR1 |
| Abbre | ESR1 |
| Synonyms | NR3A, nuclear receptor subfamily, Nuclear Receptor Subfamily 3 Group A Member, member, ESR1, ER, ESR, ESRA, ESTRR, Era, NR3A1, ER alpha, ER-Alpha, Estradiol Receptor, Estrogen Receptor, Estrogen Receptor α, group A, member 1, nuclear receptor subfamily 3, Nuclear Receptor Subfamily 3 Group A Member 1, 7*/654 isoform, 7*/819 2 isoform, 7*/822 isoform, 8*/901 isoform, 8*/941 isoform, DKFZp686N23123, Estrogen nuclear receptor alpha, Estrogen receptor 1, Estrogen receptor alpha, Estrogen receptor alpha 3*, Estrogen receptor alpha delta 3*, Estrogen receptor alpha delta 4 +49 isoform, Estrogen receptor alpha E1 E2 1 2, Estrogen receptor alpha E1 N2 E2 1 2 |
| Swissprot | |
| Calculated MW | 66 kDa |
| Observed MW |
79 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, Cytoplasm, Cell membrane |
| Tissue Specificity | Widely expressed. Isoform 3 is not expressed in the pituitary gland. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Neuroscience, Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | 3P11 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. |
Other Clones
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