Recombinant ECH1 Monoclonal Antibody (AN300278P)
For research use only.
| Verified Samples | Verified Samples in WB: HepG2, Jurkat, A549 |
| Dilution | WB 1:500-1:2000, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human ECH1 Protein |
| Abbre | ECH1 |
| Synonyms | ECH, Delta, 5)-Delta, ECH1, HPXEL, 4)-dienoyl-CoA isomerase, 5)-Delta(2, Delta(3, mitochondrial, Delta(3 5) Delta(2 4) dienoyl CoA isomerase, Delta(3 5) Delta(2 4) dienoyl CoA isomerase mitochondrial, Delta3 5 delta2 4 dienoyl CoA isomerase, Dienoyl CoA isomerase, ECH 1, Enoyl Coenzyme A hydratase 1, Enoyl Coenzyme A hydratase 1 peroxisomal, MS1034, Peroxisomal enoyl CoA hydratase 1, Peroxisomal enoyl coenzyme A hydratase like protein |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW |
36 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Tags & Cell Markers, Signal Transduction, Cardiovascular, Metabolism |
| Clone No. | 14B7 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,1-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. |
Other Clones
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Unconjugated
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