Recombinant E-Selectin/CD62e/SELE Monoclonal Antibody (AN300321P)
For research use only.
| Verified Samples | Verified Samples in WB:?Jurkat |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human CD62E protein |
| Abbre | SELE |
| Synonyms | LYAM, CD62E and ELAM, Endothelial adhesion molecule, Endothelial leukocyte adhesion molecule, Leukocyte-endothelial cell adhesion molecule, LECAM, SELE, CD62E, ELAM, ELAM1, ESEL, LECAM2, CD62E and ELAM1, E selectin, ELAM-1, Endothelial adhesion molecule 1, E-selectin (SELE), Leukocyte-endothelial cell adhesion molecule 2, Selectin E, E-selectin, LYAM2, CD26E, Endothelial leukocyte adhesion molecule 1, CD62 antigen-like family member E, Sialyl Lewis X, Ceramide-dodecasaccharide, ELAM-1, LECAM-2 |
| Swissprot | |
| Calculated MW | 67 kDa |
| Observed MW |
51 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Cardiovascular, Stem Cells |
| Clone No. | 8D10 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils. |
Other Clones
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Unconjugated
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