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Recombinant E.coli Beta-Glucuronidase/Uida/Beta-Gus Protein (His Tag) (PKSQ050059)

All Size Price Qty
50μg $ 198.00
10μg $ 88.00
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For research use only.

Synonyms Beta-glucuronidase, GUS, uidA
Species E.coli
Expression Host E.coli
Sequence Met1-Gln603
Accession P05804
Calculated Molecular Weight 69.9 kDa
Observed Molecular Weight 72 kDa
Tag N-His
Bio-activity Not validated for activity
Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
Reconstitution Not Applicable
Background Beta-D-glucuronidase from E.coli is a highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.
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