Recombinant DNPH1 Monoclonal Antibody (AN300765L)
For research use only.
| Verified Samples | Verified Samples in WB: K-562 |
| Dilution | WB 1:1000-5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human DNPH1 protein |
| Abbre | DNPH1 |
| Synonyms | DNPH, C6orf, dJ330M, C6orf108, RCL, dJ330M21.3, DNPH1, 2' deoxynucleoside 5' phosphate N hydrolase 1, BC048355, c Myc responsive, c Myc responsive protein Rcl, C76683, Chromosome 6 open reading frame 108, c-Myc-responsive protein Rcl, Deoxyribonucleoside 5' monophosphate N glycosidase, Deoxyribonucleoside 5''-monophosphate N-glycosidase, MGC54855, Putative c Myc responsive, RP3 330M21.3, RCL |
| Swissprot | |
| Observed MW |
19 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology |
| Clone No. | 9D3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene was identified on the basis of its stimulation by c-Myc protein. The latter is a transcription factor that participates in the regulation of cell proliferation, differentiation, and apoptosis. The exact function of this gene is not known but studies in rat suggest a role in cellular proliferation and c-Myc-mediated transformation. Two alternative transcripts encoding different proteins have been described. |
Other Clones
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Unconjugated
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