Recombinant DDR1 Monoclonal Antibody (AN301396L)
For research use only.
| Verified Samples | Verified Samples in WB: T47D |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human DDR1 protein |
| Abbre | DDR1 |
| Synonyms | RTK, HGK, MCK, PTK, EDDR, NTRK, CD167, DDR, HGK2, MCK10, PTK3, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, DDR1, CD 167, CD167 antigen-like family member A, CD167a, Cell adhesion kinase, DDR, DDR 1, Discoidin domain receptor, Discoidin domain receptor tyrosine kinase 1, Discoidin receptor tyrosine kinase, Discoidin receptor tyrosine kinase isoform a, EDDR 1, Epithelial discoidin domain receptor 1, Epithelial discoidin domain-containing receptor 1, Epithelial specific receptor kinase, Mammarian carcinoma kinase 10, Mammary carcinoma kinase 10, MCK-10, Neuroepithelial tyrosine kinase, Neurotrophic tyrosine kinase receptor type 4, NTRK 4, OTTHUMP00000029343, OTTHUMP00000029344, OTTHUMP00000029345, OTTHUMP00000029346, OTTHUMP00000029347, OTTHUMP00000164863, OTTHUMP00000164867, OTTHUMP00000222080, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, PTK 3, PTK 3A protein tyrosine kinase 3A, Receptor tyrosine kinase NEP, RTK 6, TRK E, Tyrosine kinase DDR, Tyrosine kinase receptor E, Tyrosine-protein kinase CAK |
| Swissprot | |
| Calculated MW | 101 kDa |
| Observed MW |
125 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein, Cell membrane; Single-pass type I membrane protein, Secreted, Cell membrane; Single-pass type I membrane protein. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Cancer, Cardiovascular |
| Clone No. | 11A9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
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Unconjugated
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