Recombinant Cytokeratin 5 Monoclonal Antibody (AN301017L)
For research use only.
| Verified Samples |
Verified Samples in WB: HaCat Verified Samples in IHC: Human tonsil tissue, Mouse lung tissue |
| Dilution | IHC 1:200-1:1000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Cytokeratin 5 protein |
| Abbre | Cytokeratin 5 |
| Synonyms | EBS, KRT, keratin, KRT5, CK5, DDD, DDD1, EBS2, K5, KRT5A, keratin 5, 58 kDa cytokeratin, basic, CK-5, Cytokeratin5, Cytokeratin-5, Dowling-Meara/Kobner/Weber-Cockayne types), epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, gene 5, K2C5, keratin 5 (epidermolysis bullosa simplex, keratin complex 2, Keratin5, Keratin-5, KRT 5, type II cytoskeletal 5, Type-II keratin Kb5 |
| Swissprot | |
| Calculated MW | 62 kDa |
| Observed MW |
62 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction |
| Clone No. | 2B13 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | KRT5(keratin 5) Homo sapiens The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. |
Other Clones
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Unconjugated
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