Recombinant Cynomolgus Thymic Stromal Lymphopoietin/TSLP (C-6His) (PKSQ050102)
For research use only.
| Synonyms | TSLP, Thymic stroma-derived lymphopoietin, Thymic stromal lymphopoietin |
| Species | Cynomolgus macaques |
| Expression Host | E.coli |
| Sequence | Tyr29-Gln159(Glu37Gln) |
| Accession | XP_005557555.1 |
| Calculated Molecular Weight | 16.2 kDa |
| Observed Molecular Weight | 16 kDa |
| Tag | C-His |
| Bio-activity | Loaded Anti-Human TSLP mAb-Fc on Protein A Biosensor, can bind Cynomolgus TSLP-His with an affinity constant of 0.07 nM as determined in BLI assay. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 6% Trehalose, 2% Glycine, 50mM NaCl, 0.05% Tween 80, pH7.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Thymic stromal lymphopoietin (TSLP) is a protein belonging to the cytokine family, contains 140 amino acids. It is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. TSLP induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c+ dendritic cells. It can induce allergic inflammation by directly activating mast cells. TSLP is produced mainly by non-hematopoietic cells such as fibroblasts, epithelial cells and different types of stromal or stromal-like cells. These cells are located in regions where TSLP activity is required. |
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