Recombinant COX2 Monoclonal Antibody (AN301043L)
For research use only.
| Verified Samples | Verified Samples in WB: Hela |
| Dilution | WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human COX2 protein |
| Abbre | COX2 |
| Synonyms | PHS, PGH, PTGS, PGHS, hCox, H synthase, PGH synthase, Cyclooxygenase, Prostaglandin Endoperoxide Synthase, COX, PTGS2, COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2, Prostaglandin, PGH2, G, Cyclooxygenase 2, PHS II, PGH synthase 2, Prostaglandin Endoperoxide Synthase 2, H synthase 2, COX 2, Cyclooxygenase 2b, Cyclooxygenase2, Cyclooxygenase-2, EC 1.14.99.1, fj02a10, Glucocorticoid-regulated inflammatory cyclooxygenase, Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase, hCox 2, Macrophage activation-associated marker protein P71/73, OTTHUMP00000033524, PES-2, PGHS 2, PGHS2, PHS 2, PHS2, Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), Prostaglandin G/H synthase, Prostaglandin G/H synthase 2, Prostaglandin G/H synthase 2 precursor, Prostaglandin G/H synthase and cyclooxygenase, Prostaglandin H2 synthase 2, Prostaglandin-endoperoxide synthase 2, prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), ptgs2a, TIS10, TIS10 protein, unp1239, wu:fj02a10 |
| Swissprot | |
| Calculated MW | 69 kDa |
| Observed MW |
75 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Tissue Specificity | Bone marrow, seminal vesicle, urinary bladder. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Cancer, Metabolism |
| Clone No. | 6C10 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. |
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Unconjugated
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