Recombinant CD5/Cluster of Differentiation 5 Monoclonal Antibody (AN300003P)
For research use only.
| Verified Samples | Verified Samples in WB: Jurkat, HeLa, 293T, HuT 78 |
| Dilution | WB 1:500-1:1000, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human CD5 / Cluster of Differentiation 5 Protein |
| Abbre | CD5 |
| Synonyms | LymphocyteantigenT1/Leu, Lymphocyte antigen T1/Leu, T-cellsurfaceglycoproteinCD, T-cell surface glycoprotein CD, OTTHUMP, LEU, CD5, LEU1, T1, CD 5, CD5 antigen, CD5 antigen (p56 62), CD5 molecule, Leu-1, Ly-1, LyA, LymphocyteantigenT1/Leu-1, OTTHUMP00000236973, T-cellsurfaceglycoproteinCD5, Tp67, T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, Tp67, Leu-1, Ly-1, CD 5, CD5, CD5 antigen (p56 62), CD5 antigen, CD5 molecule, CD5, LEU1, LyA, Lymphocyte antigen T1/Leu-1, OTTHUMP00000236973, T-cell surface glycoprotein CD5, T1 |
| Swissprot | |
| Calculated MW | 55 kDa |
| Observed MW |
58 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Stem Cells |
| Clone No. | 1A7 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | CD5, also known as Leu-1, is a type I transmembrane protein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is expressed on thymocytes, T cells and the B1a subpopulation of B cells. CD5 binds the C-type lectin, CD72, and modulates signaling through the antigen receptors on T and B cells. CD5 has been shown to provide either positive or negative co-stimulatory signals depending on cell type to regulate immune responses. |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
