Recombinant CD47 Monoclonal Antibody (AN300576P)
For research use only.
| Verified Samples |
Verified Samples in WB:?Mouse brain Verified Samples in IP: Mouse brain |
| Dilution | WB 1:500-1:2000, IP 1-5 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Mouse |
| Applications | WB, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Mouse CD47 Protein |
| Abbre | CD47 |
| Synonyms | MER, Protein MER, Leukocyte Surface Antigen CD, Antigenic Surface Determinant Protein OA, IAP, Itgp, 9130415E20Rik, B430305P08Rik, Antigenic Surface Determinant Protein OA3, Integrin-Associated Protein, LAP, Leukocyte Surface Antigen CD47, MER6, OA3, Protein MER6, Cd47 |
| Swissprot | |
| Calculated MW | 40 kDa |
| Observed MW |
40 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Clone No. | 11A3 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Predicted to enable protein binding activity involved in heterotypic cell-cell adhesion and thrombospondin receptor activity. Involved in regulation of nitric oxide biosynthetic process. Acts upstream of or within several processes, including monocyte aggregation; opsonization; and positive regulation of phagocytosis. Located in extracellular exosome. Is integral component of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; hemolymphoid system gland; and liver and biliary system. Orthologous to human CD47 (CD47 molecule). |
Other Clones
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Other Formats
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Unconjugated
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