Recombinant CD45 Monoclonal Antibody (AN300907L)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat Verified Samples in IHC: Human tonsils |
| Dilution | IHC 1:1000-1:10000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human CD45 protein |
| Abbre | CD45 |
| Synonyms | B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, PTPRC, CD45 antigen, CD45R0, Ly-5, protein tyrosine phosphatase, receptor type, Receptor-type tyrosine-protein phosphatase C, CD45RO, Receptor-type tyrosine-protein phosphatase CLeukocyte common antigen (L-CA), T200, c polypeptide, CD 45, homolog of, Leukocyte common antigen, loc, Lyt-4, OTTHUMP00000033813, OTTHUMP00000033816, OTTHUMP00000033817, OTTHUMP00000038574, Protein tyrosine phosphatase receptor type c polypeptide, protein tyrosine phosphatase, receptor type, C, receptor type C, T200 glycoprotein, T200 leukocyte common antigen, B220, CD45, CD45 antigen, CD45R, GP180, L-CA, LCA, Ly-5, LY5, Protein tyrosine phosphatase, receptor type, Ptprc, Receptor-type tyrosine-protein phosphatase C, T200 |
| Swissprot | |
| Calculated MW | 147 kDa |
| Observed MW |
240-200 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Immunology, Signal Transduction, Neuroscience, Stem Cells, Cardiovascular |
| Clone No. | 8C8 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. |
Other Clones
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Other Formats
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Unconjugated
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