Recombinant CD4 Monoclonal Antibody (AN300792L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse thymus |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human CD4 protein |
| Abbre | CD4 |
| Synonyms | Scd, T-cell surface antigen T4/Leu, T-cell surface glycoprotein CD, CD4, CD4mut, p55, Scd4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, T4, Leu-3, L3T4, W3/25, CD 4, CD4 (L3T4), CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4+ Lymphocyte deficiency, included, L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, MGC165891, OTTHUMP00000238897, T cell antigen T4, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T cell OKT4 deficiency, T cell surface antigen T4/Leu 3, T cell surface antigen T4/Leu3, T cell surface glycoprotein CD4, W3/25, W3/25 antigen |
| Swissprot | |
| Calculated MW | 51 kDa |
| Observed MW |
60 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Immunology, Stem Cells |
| Clone No. | 3G2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | CD4 molecule(CD4) Homo sapiens This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. |
Other Clones
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Other Formats
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Unconjugated
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