Recombinant CD32b/FCGR2B Monoclonal Antibody (AN300310P)
For research use only.
| Verified Samples |
Verified Samples in WB:?Daudi, THP1 Verified Samples in IHC: Human lymphonode |
| Dilution | WB 1:500-1:1000, IHC-P 1:2500-1:15000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human CD32b protein |
| Abbre | FCGR2B |
| Synonyms | FCG, CDw, Fcgr, IGFR, FCGR2B, CD32, CD32B, FCG2, FCGR2, IGFR2, CDw32, Fc-gamma-RIIb, FcRII-b, Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, Fc-gamma RII-b, CD32 antigen, Fc fragment of IgG, Fc gamma receptor IIB, Fc gamma RII, Fc gamma RIIB, FCG2B, Fc-gamma RII, FCRII, FcγRⅡ, IgG Fc receptor II beta, low affinity II, low affinity IIb, Low affinity immunoglobulin gamma Fc region receptor II, Ly-17, Lym 1, Lymphocyte antigen 17, receptor, receptor (CD32), receptor for, receptor for (CD32) |
| Swissprot | |
| Calculated MW | 34 kDa |
| Observed MW |
38 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Stem Cells |
| Clone No. | 7F12 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Fc gamma RII, also known as CD32, is a group of three closely related proteins (Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIC) that share greater than 94% amino acid identity in their extracellular domains with Fc gamma RIIB and Fc gamma RIIC having identical extracellular domains. They function as transmembrane receptors for the Fc portion of IgG molecules. These proteins are expressed by various hematopoietic cells including monocytes, macrophages, neutrophils, NK, T and B cells. The Fc gamma RII proteins are involved in phagocytosis of immune complexes and modulation of antibody production by B cells. |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
